Letter from C. S. Newhall to John Muir, 1902 Jan 6.

[letterhead]Fresno, Cali. Jan. 6, 1902.Mr. John MuirMartinez, Cal...Dear Sir,I thank you very sincerely for your telegram of the 4th to the Commission. In the campaign last fall, I refused to allow the officers under me to leave there legitimate work for political work, & declined a demand for campaign money. Also a supervisor who was a political friend of a Congressman was discharged for inefficiency, or worse. And there was a charge I cannot account for that I had antagonized the Water & [illegible] Association. Under the circumstances I thought it allowable to write & telegraph as I did to you & Wm. Thomas, & Warren Olney & Pres. Jordan. The [illegible] was more than I anticipated. Very sincerely yours,C. S. Newhall [illegible] Supt.I enclose 1.10 for the telegram.0292

Pathogen-host reorganization during Chlamydia invasion revealed by cryo-electron tomography

Invasion of host cells is a key early event during bacterial infection, but the underlying pathogen-host interactions are yet to be fully visualised in three-dimensional detail. We have captured snapshots of the early stages of bacterial-mediated endocytosis in situ by exploiting the small size of chlamydial elementary bodies (EBs) for whole cell cryo-electron tomography. Chlamydiae are obligate intracellular bacteria that infect eukaryotic cells and cause sexually transmitted infections and trachoma, the leading cause of preventable blindness. We demonstrate that Chlamydia trachomatis LGV2 EBs are intrinsically polarised. One pole is characterised by a tubular inner membrane invagination, while the other exhibits asymmetric periplasmic expansion to accommodate an array of type III secretion systems (T3SSs). Strikingly, EBs orient with their T3SS-containing pole facing target cells, enabling the T3SSs to directly contact the cellular plasma membrane. This contact induces enveloping macropinosomes, actin-rich filopodia and phagocytic cups to zipper tightly around the internalising bacteria. Once encapsulated into tight early vacuoles, EB polarity and the T3SSs are lost. Our findings reveal previously undescribed structural transitions in both pathogen and host during the initial steps of chlamydial invasion

Three-Body Dynamics with Gravitational Wave Emission

We present numerical three-body experiments that include the effects of gravitational radiation reaction by using equations of motion that include the 2.5-order post-Newtonian force terms, which are the leading order terms of energy loss from gravitational waves. We simulate binary-single interactions and show that close approach cross sections for three 1 solar mass objects are unchanged from the purely Newtonian dynamics except for close approaches smaller than 1.0e-5 times the initial semimajor axis of the binary. We also present cross sections for mergers resulting from gravitational radiation during three-body encounters for a range of binary semimajor axes and mass ratios including those of interest for intermediate-mass black holes (IMBHs). Building on previous work, we simulate sequences of high-mass-ratio three-body encounters that include the effects of gravitational radiation. The simulations show that the binaries merge with extremely high eccentricity such that when the gravitational waves are detectable by LISA, most of the binaries will have eccentricities e > 0.9 though all will have circularized by the time they are detectable by LIGO. We also investigate the implications for the formation and growth of IMBHs and find that the inclusion of gravitational waves during the encounter results in roughly half as many black holes ejected from the host cluster for each black hole accreted onto the growing IMBH.Comment: 34 pages, 14 figures, minor corrections to match version accepted by Ap

Probabilistic Volcanic Hazard and Risk Assessment

No abstrac

Menelusuri Kebenaran Letusan Gunung Merapi 1006

Http://dx.doi.org/10.17014/ijog.vol1no4.20064Until now, the large eruption of Merapi in 1006 is believed to take place although the truth is still debatable. Previous investigation proposed that the ”pralaya” of the Ancient Mataram Kingdom in 928 Saka (1006) was due to a volcanic activity. Bemmelen also inferred that impact of the eruption had destroyed and covered the Mendut and Borobudur Temples and dammed the Progo River. However, if the “pralaya” was caused by Merapi eruption, why the deposit that correlates to the the eruption is not recognized. If so, the eruption that covered the temples should have been very large, and left deposits around Merapi and of course easy to find. Historically, the “pralaya“ mentioned in the Pucangan Inscription did not happen in 1006, but in 1016 or 1017. However the “pralaya“ was caused by the attack of King Wurawari, not by the Merapi eruption. According to the history of Merapi eruptions, 11 large eruptions have occurred since 3000 years ago. However, none of those fi t with 1006 eruption. Except the large eruption (VEI 3-4), that produced Selo tephra, dated 1112 ± 73 years BP (765-911)

Internal tidal modal ray refraction and energy ducting in baroclinic Gulf Stream currents

Author Posting. © American Meteorological Society, 2018. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 48 (2018): 1969-1993, doi:10.1175/JPO-D-18-0031.1.Upstream mean semidiurnal internal tidal energy flux has been found in the Gulf Stream in hydrodynamical model simulations of the Atlantic Ocean. A major source of the energy in the simulations is the south edge of Georges Bank, where strong and resonant Gulf of Maine tidal currents are found. An explanation of the flux pattern within the Gulf Stream is that internal wave modal rays can be strongly redirected by baroclinic currents and even trapped (ducted) by current jets that feature strong velocities above the thermocline that are directed counter to the modal wavenumber vector (i.e., when the waves travel upstream). This ducting behavior is analyzed and explained here with ray-based wave propagation studies for internal wave modes with anisotropic wavenumbers, as occur in mesoscale background flow fields. Two primary analysis tools are introduced and then used to analyze the strong refraction and ducting: the generalized Jones equation governing modal properties and ray equations that are suitable for studying waves with anisotropic wavenumbers.The Woods Hole research was supported by National Science Foundation Grant OCE-1060430 and by the Office of Naval Research Grants N00014-11-1-0701 and N00014-17-1-2624. The USM research was supported by ONR Grant N00014-15-1-2288 and National Science Foundation Grant OCE-1537449.2019-02-2

Equations of motion according to the asymptotic post-Newtonian scheme for general relativity in the harmonic gauge

The asymptotic scheme of post-Newtonian approximation defined for general relativity (GR) in the harmonic gauge by Futamase & Schutz (1983) is based on a family of initial data for the matter fields of a perfect fluid and for the initial metric, defining a family of weakly self-gravitating systems. We show that Weinberg's (1972) expansion of the metric and his general expansion of the energy-momentum tensor ${\bf T}$, as well as his expanded equations for the gravitational field and his general form of the expanded dynamical equations, apply naturally to this family. Then, following the asymptotic scheme, we derive the explicit form of the expansion of ${\bf T}$ for a perfect fluid, and the expanded fluid-dynamical equations. (These differ from those written by Weinberg.) By integrating these equations in the domain occupied by a body, we obtain a general form of the translational equations of motion for a 1PN perfect-fluid system in GR. To put them into a tractable form, we use an asymptotic framework for the separation parameter $\eta$, by defining a family of well-separated 1PN systems. We calculate all terms in the equations of motion up to the order $\eta ^3$ included. To calculate the 1PN correction part, we assume that the Newtonian motion of each body is a rigid one, and that the family is quasi-spherical, in the sense that in all bodies the inertia tensor comes close to being spherical as $\eta \to 0$. Apart from corrections that cancel for exact spherical symmetry, there is in the final equations of motion one additional term, as compared with the Lorentz-Droste (Einstein-Infeld-Hoffmann) acceleration. This term depends on the spin of the body and on its internal structure.Comment: 42 pages, no figure. Version accepted for publication in Physical Review

Dynamic Anchoring of PKA Is Essential during Oocyte Maturation

SummaryIn the final stages of ovarian follicular development, the mouse oocyte remains arrested in the first meiotic prophase, and cAMP-stimulated PKA plays an essential role in this arrest. After the LH surge, a decrease in cAMP and PKA activity in the oocyte initiates an irreversible maturation process that culminates in a second arrest at metaphase II prior to fertilization [1]. A-kinase anchoring proteins (AKAPs) mediate the intracellular localization of PKA and control the specificity and kinetics of substrate phosphorylation [2]. Several AKAPs have been identified in oocytes including one at 140 kDa [3, 4] that we now identify as a product of the Akap1 gene. We show that PKA interaction with AKAPs is essential for two sequential steps in the maturation process: the initial maintenance of meiotic arrest and the subsequent irreversible progression to the polar body extruded stage. A peptide inhibitor (HT31) that disrupts AKAP/PKA interactions stimulates oocyte maturation in the continued presence of high cAMP. However, during the early minutes of maturation, type II PKA moves from cytoplasmic sites to the mitochondria, where it associates with AKAP1, and this is shown to be essential for maturation to continue irreversibly

Instrumentation for open ocean aquaculture monitoring

The Woods Hole Oceanographic Institution is assisting the University of New Hampshire by instrumenting a fish cage and mooring as part of their Open Ocean Aquaculture demonstration program in the Gulf of Maine. To understand these systems, the wave and current forcing and the response of the mooring and fish cage needs to be measured. A UNH mooring with an ADCP measured the current forcing. Tension in the mooring lines was measured by load cells deployed with the mooring during servicing in August 2000. Load cells were placed in each anchor line, and, in the NE corner, also in the two grid lines and the riser line to the fish cage. Low power recording systems were deployed on the load cell mounting bars by divers on 22 October 2000, recorded good data through January 2001, when they were turned around and redeployed. Three single load cell recorders were recovered in July 2001 and recorded though 23 June when their data storage filled. The four load cell system was recovered in March after a large winter storm, and had failed in early March. The wave forcing was measured with a wave rider buoy with a 3-axis accelerometer measuring its motion. The acceleration was integrated twice to obtain wave displacement. The system mooring contained a compliant elastic. The wave rider was deployed on 4 January 2001 and recovered on 17 March 2001 after a major Northeast storm. It recorded data throughout its deployment. The motion of the moored fish cage was measured by a motion package constructed around a 6-axis Motion-Pak and a PC-104 data system. The motion package was deployed on the fish cage from Jan into March 2001 and recorded motions thoughout without difficuly. It observed a major storm in early March where the counter weight was lost from the fish cage, and its increase in motion thereafter.Fudning was provided by National Oceanic and Atmospheric Adminstration for the Open Ocean Aquaculture Project under Contract No. NA86RG0016 to the University of New Hampshire and under Subcontracts 00-394 and 01-442 to the Woods Hole Oceanographic Institution